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quantitative real time pcr  (Toyobo)


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    Toyobo quantitative real time pcr
    Quantitative Real Time Pcr, supplied by Toyobo, used in various techniques. Bioz Stars score: 99/100, based on 6252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thunderbird+quantitative+real+time+pcr+mix/THUNDERBIRD+Probe+qPCR+Mix/pmc13130484-73-0-11
    Average 99 stars, based on 6252 article reviews
    quantitative real time pcr - by Bioz Stars, 2026-11
    99/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Potential role of lipophagy impairment for anticancer effects of glycolysis-suppressed pancreatic ductal adenocarcinoma cells
    Article Snippet: Total RNA was isolated from PANC-1 cells using Sepasol-RNA I reagent (Nacalai Tesque) and reverse-transcribed using ReverTra Ace qPCR RT Master Mix (TOYOBO, Osaka, Japan). .. The resulting cDNA was mixed with THUNDERBIRD quantitative real-time PCR mix (TOYOBO). ..

    Article Title: Evaluation of Parent- and Metabolite-Induced Mitochondrial Toxicities Using CYP-Introduced HepG2 cells.
    Article Snippet: RNA Isolation and Quantitative Real-Time PCR Total RNA was isolated on day 3 from HepG2 or TC-Hep cells using Sepasol-RNA I Super G (Nacalai Tesque), and it was reverse transcribed using ReverTra Ace qPCR RT Master mix (TOYOBO, Osaka, Japan). .. The mixture containing cDNA, primers, and THUNDERBIRD quantitative real-time PCR mix (TOYOBO) was subjected to quantitative real-time PCR using a LightCycler 96 Real-Time PCR System (Roche, Basel, Switzerland). ..

    Article Title: New in vitro screening system to detect drug-induced liver injury using a culture plate with low drug sorption and high oxygen permeability.
    Article Snippet: Drug-induced liver injury (DILI) is a major factor underlying drug withdrawal from the market.. Therefore, it is important to predict DILI during the early phase of drug discovery.. Metabolic activation and mitochondrial toxicity are good indicators of the potential for DILI.



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    PARsylation of TFEB is crucial for its interaction with TCF-1/LEF-1 and Wnt-TFEB target gene expression. (A) TFEB with mutations at residues 237K and 274K is not PARsylated by TNKS1. (B, C) TFEB with mutations in the PARsylation sites does not bind to HA-TCF-1 (B) or HA-LEF-1 (C) in HEK293T cells. (D) The self-interaction of the TFEB mutant, which has mutations at PARsylation sites, was not reduced by the treatment of Wnt3a-CM. HEK293A cells were transfected with TFEB mutants carrying different tags and treated with Wnt3a-CM for 2 hours. (E) Treatment of HEK293A cells with Wnt3a-CM for 2 hours does not reduce the level of oligomer of TFEB PARsylation-defective mutants. (F) PARsylation of TFEB is necessary for Wnt-TFEB target gene expression. HEK293A cells were knocked down and transfected with the indicated constructs, then treated with Wnt3a-CM for 8 hours. The cells were then used <t>for</t> <t>real-time</t> <t>PCR.</t> Statistical analysis was performed using a 2-tailed unpaired t-test, showing exact P- values in each figure. Error bars indicate the standard deviation of triplicate measurements (technical replicates).
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    PARsylation of TFEB is crucial for its interaction with TCF-1/LEF-1 and Wnt-TFEB target gene expression. (A) TFEB with mutations at residues 237K and 274K is not PARsylated by TNKS1. (B, C) TFEB with mutations in the PARsylation sites does not bind to HA-TCF-1 (B) or HA-LEF-1 (C) in HEK293T cells. (D) The self-interaction of the TFEB mutant, which has mutations at PARsylation sites, was not reduced by the treatment of Wnt3a-CM. HEK293A cells were transfected with TFEB mutants carrying different tags and treated with Wnt3a-CM for 2 hours. (E) Treatment of HEK293A cells with Wnt3a-CM for 2 hours does not reduce the level of oligomer of TFEB PARsylation-defective mutants. (F) PARsylation of TFEB is necessary for Wnt-TFEB target gene expression. HEK293A cells were knocked down and transfected with the indicated constructs, then treated with Wnt3a-CM for 8 hours. The cells were then used for real-time PCR. Statistical analysis was performed using a 2-tailed unpaired t-test, showing exact P- values in each figure. Error bars indicate the standard deviation of triplicate measurements (technical replicates).

    Journal: Molecules and Cells

    Article Title: Tankyrase-1-mediated PARsylation directs TFEB partner switching to regulate selective Wnt target gene expression

    doi: 10.1016/j.mocell.2026.100313

    Figure Lengend Snippet: PARsylation of TFEB is crucial for its interaction with TCF-1/LEF-1 and Wnt-TFEB target gene expression. (A) TFEB with mutations at residues 237K and 274K is not PARsylated by TNKS1. (B, C) TFEB with mutations in the PARsylation sites does not bind to HA-TCF-1 (B) or HA-LEF-1 (C) in HEK293T cells. (D) The self-interaction of the TFEB mutant, which has mutations at PARsylation sites, was not reduced by the treatment of Wnt3a-CM. HEK293A cells were transfected with TFEB mutants carrying different tags and treated with Wnt3a-CM for 2 hours. (E) Treatment of HEK293A cells with Wnt3a-CM for 2 hours does not reduce the level of oligomer of TFEB PARsylation-defective mutants. (F) PARsylation of TFEB is necessary for Wnt-TFEB target gene expression. HEK293A cells were knocked down and transfected with the indicated constructs, then treated with Wnt3a-CM for 8 hours. The cells were then used for real-time PCR. Statistical analysis was performed using a 2-tailed unpaired t-test, showing exact P- values in each figure. Error bars indicate the standard deviation of triplicate measurements (technical replicates).

    Article Snippet: Quantitative real-time PCR (qPCR) was conducted using THUNDERBIRD SYBR qPCR Mix (Toyobo, QPS-201) in accordance with the manufacturer’s instructions.

    Techniques: Targeted Gene Expression, Mutagenesis, Transfection, Construct, Real-time Polymerase Chain Reaction, Standard Deviation